Fig. 3

Validation of the eight selected tsRNAs and to assess their predictive efficiency for distinguishing NOA patients with spermatogenic failure from OA patients. A Validation of the eight selected tsRNAs by qRT-PCR from NOA and OA. B ROC curve of the eight selected tsRNAs for predictive classification of azoospermic patients into NOA and OA subtypes. C Validation of the eight selected tsRNAs by qRT-PCR from NOA, IO and healthy samples. Normalized expression levels relative to the U6 gene. Data shown as the means (10–90% percentile). Statistical analysis was performed by using two-tailed unequal variant Student’s t-test (*P-value < 0.05; **P-value < 0.01; ***P-value < 0.001)