The fatty acid composition in follicles is related to the developmental potential of oocytes up to the blastocyst stage: a single-centre cohort study

Table 3 Comparison of BMI, age and proportions of specific FF FA and FA groups of the least (1st quartile, Q1) and best (4th quartile, Q4) performing quartiles, based on the assisted reproductive technology outcome parameters related to embryos developing between days 3 and 5 relative to oocytes with two pronuclei (ED3-5/2PN), expanded blastocysts on day 5 relative to oocytes with two pronuclei (EB5/2PN) and embryo utilization rate (EUR)

Item	ED3-5/2PN				EB5/2PN				EUR
	Q1	Q4	Statistics^a	P-value	Q1	Q4	Statistics¹	P-value	Q1	Q4	Statistics¹	P-value
Proportion, %	≤ 38.8	≥ 77.4			= 0.00	≥ 48.5			≤ 21.8	≥ 59.2
Patient, n	34	34			39	32			34	34
Body mass index, kg/m²	23.6	25.6	MW	0.115	24.0	24.3	MW	0.885	24.2	25.7	MW	0.136
Age, year	34.4	32.3	T	0.046	33.0	30.0	MW	0.056	32.8	31.0	T	0.048
Fatty acid (FA), % by weight
C16:0	20.9	21.0	T	0.729	20.7	21.0	T	0.471	20.7	20.9	T	0.579
C16:1n-7	1.13	1.17	T-log	0.674	1.10	1.12	T-log	0.865	1.11	1.14	T-log	0.751
C18:0	8.23	8.15	T-log	0.645	8.25	8.04	MW	0.262	8.31	8.15	T-log	0.402
C18:1n-9	16.1	16.7	T	0.214	16.4	16.6	T	0.546	16.2	16.6	T	0.384
C18:2n-6	23.9	24.1	T	0.753	23.8	24.4	T	0.396	24.3	23.7	T	0.503
C18:3n-3	0.37	0.38	MW	0.524	0.36	0.40	T-log	0.108	0.37	0.40	T-log	0.185
C20:4n-6	7.91	8.20	T	0.400	7.95	8.36	T	0.209	7.93	8.54	T	0.062
C20:5n-3	0.74	0.51	T-log	0.006	0.59	0.50	MW	0.030	0.66	0.51	T-log	0.022
C22:6n-3	2.70	2.19	T	0.002	2.48	2.15	T-log	0.003	2.50	2.22	T-log	0.037
Saturated FA	31.4	31.3	T	0.772	31.3	31.2	T	0.777	31.3	31.3	T	0.839
Monounsaturated FA	21.3	21.5	T	0.700	21.4	21.3	MW	0.954	21.2	21.4	T	0.731
n-3 Polyunsaturated FA	4.65	3.82	T-log	0.001	4.35	3.81	T-log	0.007	4.42	3.92	T-log	0.019
n-6 Polyunsaturated FA	34.8	35.4	T	0.318	34.8	35.8	T	0.145	35.2	35.3	T	0.874
n-6: n-3 Polyunsaturated FA	7.45	9.25	T-log	0.001	8.18	9.52	T	0.002	7.95	8.98	T-log	0.021

Mean, in case of normal data distribution or Median, in case of non-normal data distribution, are presented. Parameters which show significant differences (P < 0.05) or a trend (0.05 ≤ P < 0.10) are presented in bold
^aStatistics: MW Mann–Whitney U tests, T Independent T-test, T-log a T-test has been performed when log-transformed (T-log) data were normally distributed. For log-transformed data (T-log), log-back transformations were carried out to calculate the geometric mean
Sum of saturated fatty acids (Saturated FA) = Σ (C12:0, C13:0, C14:0, C15:0, C16:0, C17:0, C18:0, C20:0, C22:0, C24:0)
Sum of Monounsaturated fatty acids (Monounsaturated FA) = Σ (C14:1n-5, C16:1n-9, C16:1n-7, C18:1n-9, C18:1n-7, C20:1n-7, C20:1n-9, C22:1n-9, C24:1n-9)
Sum of n-3 Polyunsaturated fatty acids (n-3 Polyunsaturated FA) = Σ (C18:3n-3, C20:3n-3, C20:4n-3, C20:5n-3, C22:5n-3, C22:6n-3)
Sum of n-6 Polyunsaturated fatty acids (n-6 Polyunsaturated FA) = Σ (C18:2n-6, C18:3n-6, C20:2n-6, C20:3n-6, C20:4n-6, C22:4n-6, C22:5n-6)

ISSN: 1477-7827