Fig. 7

IGF2 reduces apoptosis and induces expression of known target genes in oocytes from obese mice (A) Representative images of early apoptosis labeled with Annexin V of ND, HFD, and HFD + IGF2 oocytes. Red fluorescent indicates the Annexin V positive oocytes. Scale bar = 10 μm. B The percentage of early apoptosis in MII-stage oocytes from ND (n = 100),HFD (n = 64) and HFD+ IGF2 (n = 62) group. C qRT-PCR results showing IGF2 and target genes expression in mouse MII-stage oocytes from ND, HFD, and HFD + IGF2 groups after in vitro maturation. *p < 0.05. One-way ANOVA test and students t-test (two-tailed) was used for statistical analysis. Error bars indicate the SEM