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Fig. 5 | Reproductive Biology and Endocrinology

Fig. 5

From: IGF2 reduces meiotic defects in oocytes from obese mice and improves embryonic developmental competency

Fig. 5

IGF2 increases mitochondrial activity in oocytes from obese mice (A) ND, HFD, and HFD + IGF2 oocytes were stained with JC-1 to test the mitochondrial membrane potential (MMP). Scale bar = 10 μm. B Quantification of the ratio of red to green fluorescence intensity in ND (n = 20), HFD (n = 22), and HFD + IGF2 (n = 20) oocytes. C Mitochondria were labeled with mitotracker Green FM (green). Scale bar = 20 μm. D Quantification of mitochondrial distribution signals in ND (n = 148), HFD (n = 54), and HFD + IGF2 (n = 56) oocytes. E Immunofluorescence of HPG (green) showing the overall translation levels in MII-stage ND, HFD, and HFD + IGF2 oocytes. Chromosomes were counterstained with DAPI (blue). Scale bar = 20 μm. F HPG signal intensity quantification in ND (n = 16), HFD, (n = 20) and HFD + IGF2 (n = 17) oocytes. *p < 0.05. One-way ANOVA test was used for statistical analysis. Error bars indicate the SEM

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