Fig. 3

IGF2 administration improves the embryonic developmental competency of oocytes from obese mice (A) Schematic diagram indicating treatment plan of embryos in culture medium in vitro. B Quantitative analysis of morula and blastocyst in ND (n = 72), HFD (n = 57), and HFD+ IGF2 (n = 49). C Morphology of embryos cultured in vitro and examined at the blastocyst developmental stage. The arrows point to embryos that developed into blastocysts; the arrowheads showing the embryos which failed to transform into blastocysts. Scale bar, 300 μm. D Quantification of the total and TE number of cells in ND (n = 37), HFD (n = 20) and HFD + IGF2 (n = 26) blastocysts. E Representative images of blastocysts of the ND, HFD, and HFD + IGF2 groups. The trophectoderm (TE) was stained with CDX2 (green), and chromosomes were counterstained with DAPI (blue). Scale bar, 20 μm. NS, not significant. *p < 0.05. One-way ANOVA test was used for statistical analysis. Error bars indicate the SEM