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Fig. 9 | Reproductive Biology and Endocrinology

Fig. 9

From: Diverse actions of sirtuin-1 on ovulatory genes and cell death pathways in human granulosa cells

Fig. 9

Nec-1 counteracts SIRT1 actions on pro-apoptotic and necroptotic proteins. SVOG cells were treated with either control media or SRT2104 (50 μmol/L) for 24 h or pretreated with Nec-1 (20 μmol/L) for 2 h. Protein levels (A- cleaved caspase 3, B- cleaved PARP, C-RIPK1 and D-MLKL) were determined in cell extracts by western blotting and normalized relative to the abundance of total MAPK (p44/42; loading control). Cropped image of representative western blots is shown in the upper panel. Results are presented as the means ± SEM from 3 independent experiments. The different letters indicate significant statistical differences at p < 0.05 analyzed using ANOVA followed by Tukey HSD post hoc multiple comparison test

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