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Fig. 8 | Reproductive Biology and Endocrinology

Fig. 8

From: Diverse actions of sirtuin-1 on ovulatory genes and cell death pathways in human granulosa cells

Fig. 8

Nec-1 abolishes SRT2104 actions on live and late apoptotic cell populations. SVOG cells were pretreated with Nec-1 (20 μmol/L) for 2 h, followed by incubation for 24 (B) and 48 h (D) with medium only (control) or SRT2104 (50 μmol/L). Then, cells were analyzed using FACS with propidium iodide (PI) and Annexin V staining (representative plots are shown in A-24 h and C-48 h). Results are presented as the means ± SEM from 3 independent experiments. The different letters indicate significant statistical differences at p < 0.05 analyzed using ANOVA followed by Tukey HSD post hoc multiple comparison test

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