Fig. 7

SIRT1 elevates necroptotic proteins RIPK1 (A,C) and MLKL (B,D). SVOG cells were treated with either control media or SRT2104 (50 μmol/L) for 24 h and 48 h (A and B) or cells were transfected with either scrambled siRNA (siNC) or SIRT1 siRNA (siSIRT1) and the protein was extracted at 48 h post-transfection (C and D). RIPK1 and MLKL protein levels were determined in cell extracts by specific antibodies in western blotting and normalized relative to the abundance of total MAPK (p44/42; loading control). Cropped images representative of western blot are shown in the upper panels. Results are presented as the means ± SEM from 4 independent experiments. Asterisks indicate significant differences from their respective controls (*p < 0.05, **p < 0.01)