Fig. 5

SIRT1 reduces anti-apoptotic proteins, BCL-XL and MCL1. A and B SVOG cells were treated with either control media or SRT2104 (50 μmol/L) for 24 h and 48 h. C Cells were transfected with 10 nmol/L of either scrambled siRNA (siNC) or SIRT1 siRNA (siSIRT1) and collected 48 h post transfection. BCL-XL and MCL1 protein levels were determined in cell extracts by western blotting. Cropped images representative of western blot are shown in the upper panels. Densitometric quantifications are relative to cells cultured in respective control media; protein levels were normalized to the abundance of total MAPK (p44/42; loading control). The results are presented as the means ± SEM from 3 independent experiments. Asterisks indicate significant differences from their respective controls (*p < 0.05, **p < 0.01)