Fig. 4

SRT2104 reduces live cells and increases late apoptotic and necrotic cells. A and B SVOG cells were treated with either control media or SRT2104 (50 μmol/L) or C and D cells were transfected with 10 nmol/L of either scrambled siRNA (siNC) or SIRT1 siRNA (siSIRT1). After 48 h post- treatment or transfection, cells were analyzed using FACS after propidium iodide (PI) and Annexin V staining (A and C). Results are presented as the means ± SEM from 4 and 3 independent experiments for SRT2104-treated and siSIRT1 transfected cells, respectively. E Comparison of mean cell populations between SIRT1 silencing and activation. The mean difference was calculated by subtracting the mean cell population of SRT2104- or siSIRT1- treated cells from their respective controls. Asterisks (**p < 0.01, ***p < 0.001) indicate significant differences between cells treated with SRT2104 or siSIRT1 compared to their respective controls. Hashtags (## p < 0.01, ### p < 0.001) indicate significant mean differences between cells treated with either SRT2104 vs siSIRT1