Table 1 Real-time PCR primers and sizes of the amplification products of the target genes in rat
From: The expression of IGFBP-5 in the reproductive axis and effect on the onset of puberty in female rats
Gene | Forward primer, 5′-3′ | Reverse primer, 5′-3′ | Product size,(bp) |
|---|---|---|---|
Igfbp-5 | CAGTCGTGTGGCGTCTAC | AGCGGCTTCTCCTCATCC | 77 |
Igf-1 | GCTGGTGGACGCTCTTCAGT | TTCAGCGGAGCACAGTACAT | 168 |
Gapdh | TCACCACCATGGAGAAGGC | GCTAAGCAGTTGGTGGTGCA | 134 |
Fshβ | TACTGCTACACCAGGGATCTG | TCTTACAGTGCAGTCGGTGC | 201 |
Lhβ | GCTGCTGAGCCCAAGTGT | GCTGGTGGTGAAGGTGATG | 124 |
Igf-1r | ACGCTGACCTCTGTTACCT | ATTGTTGATGGTGGTCTTCTC | 161 |
Kiss-1 | TGCTGCTTCTCCTCTGTG | CCAGGCATTAACGAGTTCC | 116 |
Akt | CTCTTCTTCCACCTGTCTCG | ACAGCCCGAAGTCCGTTA | 184 |
Mtor | GAGATACGCCGTCATTCC | GCATCAGAGTCAGGTGGTC | 176 |
Gnrh | GCCGCTGTTGTTCTGTTGAC | CTGGGGTTCTGCCATTTGA | 153 |