Fig. 9
From: The expression of IGFBP-5 in the reproductive axis and effect on the onset of puberty in female rats
The effect of IGFBP-5 on gene expression and the AKT-mTOR protein in hypothalamus cells. a Levels of Kiss-1, Gnrh, Igf-1, Tgf-β, Akt-1 and Mtor mRNA in hypothalamus cells after IGFBP-5 treatment. b Levels of AKT and mTOR protein in hypothalamus cells after IGFBP-5 treatment. c Relative gray scale of AKT expression determined following WB analysis. d Relative gray scale of mTOR expression determined following WB analysis.** indicates a very significant difference (P < 0.01); * indicates a significant difference (P < 0.05)