Fig. 5
From: Regulatory roles of alternative splicing at Ezh2 gene in mouse oocytes
Only expression of Ezh2 short isoform disrupted mitochondrial function. A Oocytes were stained with Mito-tracker (red) to show mitochondria in cytoplasm. 23 WT oocytes and 17 Ezh2Short oocytes were examined. Scale bar, 20 μm. ****P < 0.0001. B Mitochondrial membrane potentials of 10 WT and 11 Ezh2Short oocytes were detected by JC-1 staining, with histogram showing the average JC-1 aggregates/monomers fluorescence ratio. **P < 0.01. Scale bar, 50 μm. C The relative mtDNA copy number from WT and Ezh2Short oocytes (n = 20 for each group). *P < 0.05. D. ROS level of oocytes in the WT and Ezh2Short oocytes. ****P < 0.0001. Scale bar, 20 μm