Fig. 5
From: Activation of α7 nicotinic acetylcholine receptor retards the development of endometriosis
Activation of α7nAChR inhibits platelet-induced FMT in primary human endometrial stromal cells (HESCs) derived from ovarian endometrioma tissue samples. A Relative fold change in gene expression levels of α-SMA, LOX1, COL1A1 and CCN2 in HESCs treated with buffer (Con), activated platelets (PLT) with or without PNU-282987 (PNU), or PNU-282987 alone for 72 h (n = 4). B Left panel: Detection of protein levels of α-SMA and LOX1 by immunoblotting of lysate of HESCs treated with buffer (Con), activated platelets (PLT) with or without PNU-282987 (PNU), or PNU-282987 alone for 72 h. Right panel: Relative fold change of the protein levels of α-SMA and LOX1 (n = 5). C Representative immunofluorescence of SM-MHC, desmin, α-SMA and OTR expression in HESCs cells after indicated treatment for Day 12. Symbols for statistical significance levels: NS: p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001