Fig. 2

MiR-135a-5p mimics inhibit HTR-8/SVneo cell proliferation and cell cycle progression. (a) MiR-135a-5p expression in HTR-8/SVneo following miR-135a-5p mimics or NC mimics transfection. (b) MiR-135a-5p overexpression inhibited cell proliferation in CCK-8 assays, with similar results in EdU assays (c-d). (e-f) MiR-135a-5p overexpression inhibited G0/G1 to S phase progression of the cell cycle within HTR-8/SVneo cells. (g-i) Cyclin D1 and cyclin B1 protein levels were reduced following the overexpression of miR-135a-5p within HTR-8/SVneo cells, with GAPDH and β-Tubulin serving as reference controls. Outcomes are means ± SD from at least three experiments. NC: negative control; CCK-8: Cell counting Kit-8; EdU: 5-Ethynyl-2′-deoxyuridine. *P < 0.05, **P < 0.01, *** P < 0.001