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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Betaine attenuate chronic restraint stress-induced changes in testicular damage and oxidative stress in male mice

Fig. 3

Effect of betaine on spermatogenic cells in the testis of chromic stressed mice. Each bar represents the mean ± SEM (N = 6 male mice). (A) The effect of BET on the mRNA expression level of different cell markers in the testis tissue of CRS mice; (B-C) Western blot analysis the effect of BET on the protein expression level of different cell markers in the testis tissue of CRS mice, Tubulin was used as the loading control; (D) Immunohistochemical detection of INSL3 expression in testis, (D1) control group; (D2) CRS group; (D3) BET 200 mg/ kg treated group (D4) semi-quantitative statistics; *P < 0.05, compared with Control group; **P < 0.01, compared with Control group; ***P < 0.001, compared with Control group; ##P < 0.01, compared with CRS + NS group; ###P < 0.001, compared with CRS + NS group; Different symbol indicate significant difference P < 0.05 among groups determined by one-way ANOVA (followed by Bonferroni post hoc test)

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