Table 1 Primer sequences used in RT-qPCR analyses
Genes | Primer sequences (5′–3′) | Size | Ann. temp. | GenBank ID | |
|---|---|---|---|---|---|
Igf1 | F | tacctggcactctgcttgct | 194 | 64 | NM_001082477 |
R | cggaagcaacactcatccac | ||||
Lhcgr | F | ctcacctatctccctgtcaa | 365 | 64 | NM_012978 |
R | acagactcgttattcatccc | ||||
Sf1 | F | cgccaggagtttgtctgtct | 185 | 67 | NM_001191099 |
R | acctccaccaggcacaatag | ||||
Star | F | aaccaggaaggctggaagaa | 123 | 64 | NM_031558.3 |
R | tctgtccatgggctggtcta | ||||
Cyp11a1 | F | ttgcctttgagtccatcacc | 187 | 64 | NM_017286.2 |
R | gcatggtccttccaggtctt | ||||
Cyp17a1 | F | aacgttgactccagcattgg | 163 | 60 | NM_012753.2 |
R | gcgtgggtgtaatgagatgg | ||||
Hsd3b1 | F | gcattaaccccactcccact | 146 | 60 | NM_001007719.3 |
R | ggaccctgacctccttcaga | ||||
Hsd17b3 | F | gtccctggcctctttacagc | 191 | 64 | NM_008291 |
R | tttaacaaactcatcggcgg | ||||
Cyp19a1 | F | tatccggaggtggaaacagc | 181 | 60 | NM_017085 |
R | cgtcaatcacgtcatcctcc | ||||
Tnp1 | F | gatgcaagtcgcaattaccg | 184 | 67 | NM_017056.2 |
R | ccgaatttcgtcacaactgg | ||||
Tnp2 | F | gaagaccttggaagggaaagtg | 150 | 64 | NM_017057.2 |
R | tggctatctccttttgggat | ||||
Prm2 | F | tatgggaggacagaaagggg | 157 | 58 | NM_012873 |
R | tcctccttcgggatcttctg | ||||
Kiss1 | F | agctgctgcttctcctctgt | 152 | 64 | NM_181692 |
R | aggcttgctctctgcatacc | ||||
Gnrh1 | F | ccgctgttgttctgttgact | 150 | 64 | NM_012767 |
R | ggggttctgccatttgatcc | ||||
Lhb | F | tcccaggactcaaccaatga | 112 | 60 | NM_001082477 |
R | tggttagaacacctgctggc | ||||
Rpl7 | F | tcaatggagtaagcccaaag | 246 | 60 | NM_011291 |
R | caagagaccgagcaatcaag | ||||