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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Increased testicular insulin-like growth factor 1 is associated with gonadal activation by recombinant growth hormone in immature rats

Fig. 3

The effects of rhGH on HSD3B(+) and HSD17B(+) LCs in immature rat testes. A Immunohistochemistry of HSD17B in immature rat testes on PNDs 24 and 31. B Image analysis of HSD17B(+) LCs. Red cross, nuclei of LCs; black dotted line, boundary of HSD17B(+) LCs; red dotted line, boundary of blood vessels. C, D Number of HSD17B(+) LCs in each unit area (1 mm2) of the testes. E, F Mean size of HSD17B(+) LCs. G, H Total number of HSD17B(+) interstitial cells in the testes of immature rats on PNDs 24 and 31. I Immunofluorescence of HSD3B in immature rat testes on PND 24. White cross, nuclei of LCs; white dotted line, boundary of HSD3B(+) LCs; white box, area shown in the high magnification photograph. J Number of HSD3B(+) LCs in each unit area (1 mm2) of the testes. K Mean size of HSD3B(+) LCs. L Total number of HSD3B(+) interstitial cells in the testes of immature rats on PND 24. * and **, significantly different from control rats by Mann-Whitney U test at p < 0.05 and 0.01, respectively

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