Fig. 4

Effects of 10 nM rapamycin on the accumulation of DNA damage the expressions of genes associated with DDR during oocyte IVM. A The fluorescence images of DNA and γ-H2AX in oocytes matured in vivo and in vitro (with or without rapamycin). Scale bar, 20 μm. B The intensity of γ-H2AX normalized to the mean DNA intensity in three groups. C The mRNA levels of genes associated with DNA damage repair, including Atm, Atr, and Prkdc. IVV: in vivo; *P < 0.05; **P < 0.01; ***P < 0.001