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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Exosomal AFAP1-AS1 binds to microRNA-15a-5p to promote the proliferation, migration, and invasion of ectopic endometrial stromal cells in endometriosis

Fig. 3

Effect of AFAP1-AS1 binding to miR-15a-5p on the proliferation, migration and invasion ability of EcESCs. A Venn diagram of the intersection of Starbase database predicted binding miRNAs for AFAP1-AS1 and miRNA second generation sequencing analysis of differential miRNAs. B Heat map of the top 10 differential miRNAs for miRNA second generation sequencing analysis. C Predicted binding sites for AFAP1-AS1 to miR-15a-5p via LncBase. D Dual luciferase reporter gene assay to verify the targeted binding of AFAP1-AS1 to miR-15a-5p. E RIP assay to detect the enrichment of AFAP1-AS1 and miR-15a-5p in IgG and Ago2. F RNA pull down assay to detect the binding of AFAP1-AS1 to miR-15a-5p. G RT-qPCR to detect the changes of AFAP1-AS1 and miR-15a-5p expression after silencing of AFAP1-AS1. H RT-qPCR to detect the changes of miR-15a-5p expression in differently treated EcESCs. I CCK-8 to detect the changes of cell proliferation ability. J Transwell assay to detect the changes of cell migration and invasion ability. * p < 0.05 indicates the comparison between two groups; ns p > 0.05 indicates the comparison between two groups

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