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Fig. 1 | Reproductive Biology and Endocrinology

Fig. 1

From: Exosomal AFAP1-AS1 binds to microRNA-15a-5p to promote the proliferation, migration, and invasion of ectopic endometrial stromal cells in endometriosis

Fig. 1

Identification of Exo and expression of AFAP1-AS1 in EcESCs and Exo. A Expression of AFAP1-AS1 in EMS patients and non-EMS patients in microarray GSE7846. B RT-qPCR to detect the expression of AFAP1-AS1 in normal endometrial tissues (n = 20), EMS eutopic endometrial tissues (n = 30), and ectopic endometrial tissues (n = 30). C TEM to observe morphology of Exo. D NTA to determine the particle size of normal and ESC-Exo. E Western blot analysis to detect protein expression of CD9, CD63, CD81, Tsg101, and Calnexin. F RT-qPCR to study AFAP1-AS1 in Normal and EcESCs-Exo. G Fluorescence microscopy to assess of PKH67-labelled Exo internalization in EcESCs. H RT-qPCR to detect AFAP1-AS1 expression in Exo after silencing AFAP1-AS1 in EcESCs. I RT-qPCR to monitor the AFAP1-AS1 expression in EcESCs after co-culture of Exo with EcESCs. * p < 0.05 indicates the comparison between two groups; ns p > 0.05 indicates the comparison between two groups

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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