Fig. 2

Effects of BMP2 on the expression and accumulation of CTGF in hGL cells. A and B Primary hGL cells were treated with Ctrl or different concentrations (1, 10, or 100 ng/ml) of BMP2 for 24 h. The protein levels of CTGF were examined using western blot analysis (A), and the accumulated levels of CTGF in conditioned medium were examined using ELISA (B). C and D SVOG cells were treated with Ctrl or different concentrations (1, 10 or 100 ng/ml) of BMP2 for 24 h, and the mRNA (C) and protein (D) levels of CTGF were examined using RT-qPCR and western blot analysis, respectively. E and F SVOG cells were treated with Ctrl or BMP2 (50 ng/ml) at different time points (0.5, 1, 3, 6, 12 or 24 h), and the mRNA (E) and protein (F) levels of CTGF were examined using RT-qPCR and western blot analysis, respectively. The results are expressed as the mean ± SEM of at least 3 independent experiments, and values without common letters are significantly different (P < 0.05)