Fig. 1

Effects of BMP2 on the accumulated levels of hyaluronan and the expression of hyaluronan synthases in human granulosa-lutein (hGL) cells. A-D Primary hGL cells were treated with a vehicle control (Ctrl) or different concentrations (1, 10, or 100 ng/ml) of BMP2 for 24 h. The accumulated levels of hyaluronan in conditioned medium were examined using enzyme-linked immunosorbent assay (ELISA) (A), and the mRNA levels of three hyaluronan synthases (HAS1, HAS2 and HAS3) were examined using RT-qPCR (B-D). E SVOG cells were treated with Ctrl or different concentrations (1, 10, or 100 ng/ml) of BMP2 for 24 h, and the mRNA levels of HAS2 were examined using RT-qPCR. F, SVOG cells were treated with Ctrl or BMP2 (50 ng/ml) at different time points (0.5, 1, 3, 6, 12 or 24 h), and the mRNA levels of HAS2 were examined using RT-qPCR. The results are expressed as the mean ± SEM of at least 3 independent experiments, and values without common letters are significantly different (P < 0.05). Letters are used to denote the statistically significant differences between variables. If the letters on the column bar of two groups are the same (E.g. “a” vs. “a” or “b” vs. “b”), it means that there is no statistical difference between two groups. On the other hand, if the letters on the column bar of two groups are different (E.g. “a” vs. “b” or “b” vs. “c”), it means that there is significant difference between two groups. BMP2, bone morphogenetic protein 2; Ctrl, control; h, hour