Fig. 1

Expression of inflammasomes in n/eEM and OE tissues. (A) Relative mRNA expression level of NLRP3 was quantified using qRT-PCR. Expression levels are shown relative to GAPDH. Data are shown as mean ± SEM of samples obtained from patients and assayed in duplicate; nEM (n = 10), eEM (n = 12), and OE (n = 10). (B) The NLRP3 protein levels in n/eEM and OE tissues were assessed using western blot. β-actin was used as a loading control. These results are representative of three separate experiments. (C) Relative protein levels of NLRP3 were quantified. Protein levels are shown relative to those of β-actin. Data are shown as mean ± SEM of samples obtained from patients; nEM (n = 3), eEM (n = 4), and OE (n = 4). (D) Relative mRNA expression levels of NLRP3, NLRP1, NLRC4, and AIM2 were quantified. Expression levels are shown relative to those of GAPDH. Data are shown as mean ± SEM of assays conducted in duplicate from samples obtained from patients; eEM (n = 6) and OE (n = 5). Statistical analyses were conducted using one-way ANOVA followed by Dunnett’s multiple comparison test. *P < 0.05; **P < 0.01; n.s., not significant; n/eEM, eutopic endometrium without/with endometriosis, OE ovarian endometriosis