Fig. 6

G6PC overexpression promotes CC progression via AKT/mTOR pathway. (A) Protein association network of G6PC that were generated with Search Tool for the Retrieval of Interacting Genes (STRING: https://string-db.org/). (B) G6PC-related KEGG pathways in CC tissue in String database. (C) Western blot analysis of t-AKT/p-AKT((Ser473), t-mTOR/p-mTOR, t-S6/p-S6 and t-4EBP1/p-4EBP1 in the constructed cells. β-Actin was used as the loading control. The protein expression levels of indicated genes were also analysed in G6PC overexpressed cells treated with PI3K inhibitor LY294002 (20 μmol/l). (D) Western blot analysis of PKM2 and ALDOB in the constructed cells. β-Actin was used as the loading control. The protein expression levels of indicated genes were also analysed in G6PC overexpressed cells treated with PI3K inhibitor LY294002 (20 μmol/l). (E–G) Representative images showing the colony formation (D) Migration assay (E) and Tube formation assay (F) of Hela and Siha cells overexpressing G6PC or treated with LY294002