Fig. 5

G6PC promotes tumour angiogenesis in vitro and in vivo. (A) Tube formation assay was performed in Hela and Siha cells. ^*P < 0.05, ^**P < 0.01. (B) The HUVEC migration experiment was used to detect the effect of differential expression of G6PC on the migration ability of vascular endothelial cells. NC(negative control): serum-free culture medium; PC(positive control): whole serum culture medium. ^*P < 0.05, ^**P < 0.01. (C) Western blot analysis of VEGF and HIF-1α in the constructed Hela and Siha cells. β-Actin was used as the loading control. (D) CAM assays were performed to confirm the effect of G6PC on tumour angiogenesis ex vivo. ^*P < 0.05, ^**P < 0.01