Fig. 3
From: Homozygous SPAG6 variants can induce nonsyndromic asthenoteratozoospermia with severe MMAF
Lower expression of SPAG6 in spermatozoa from men harboring SPAG6 variants. A Immunofluorescence analysis: SPAG6 staining (red) was located along entire the sperm flagella from a normal control, while SPAG6 staining was extremely weak and discontinuous in the sperm flagella from F1 II-1 and F2 II-1. The anti-acetylated tubulin staining (green) was used as a flagellar maker. Scale bar: 20 µm. B SPAG6 protein levels were determined using western blotting in spermatozoa from F1 II-1, F2 II-1 and two healthy controls. Beta-actin was used as loading control