Fig. 6
From: FTO protects human granulosa cells from chemotherapy-induced cytotoxicity
Overexpression of FTO attenuated injured granulosa cells’ apoptosis by downregulating the expression of BNIP3. A Compared to the NC group, the BNIP3 mRNA level was decreased in the FTO group. B The western blotting result showed that overexpression of FTO could reverse the expression level of BNIP3 induced by cisplatin. C qRT-PCR showed that downregulation of FTO increased the expression level of BNIP3. D The western blotting showed that downregulation of FTO promoted the expression level of BNIP3 induced by cisplatin. β-actin served as the loading control. One representative experiment of three independent experiments is shown. The results represent the mean ± SEM from three independent experiments. Student’s t-test and one-way ANOVA were applied to compare the two experimental and multiple groups, respectively. (*P < 0.05, **P < 0.01, ***P < 0.001, ns, no significance)