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Fig. 5 | Reproductive Biology and Endocrinology

Fig. 5

From: FTO protects human granulosa cells from chemotherapy-induced cytotoxicity

Fig. 5

Down-regulation of FTO promoted injured granulosa cells apoptosis. A The transfection efficiency of si-FTO was measured by qRT-PCR. B-C The mRNA expression of Bcl-2 and BAX in transfected granulosa cells was assessed using qRT-PCR and normalized to GAPDH. D-G The protein expression of FTO, BAX, and Bcl-2 was determined by western blotting with β-actin as a loading control in different groups. (H-K) Western blotting results showed that inhibit FTO expression in injured granulosa cells could enhance the cisplatin-induced change of FTO, Bcl-2, and BAX. L-M EdU labeling and cck-8 assay indicated that cells viability was inhibited in cisplatin-induced injured cells, and co-transfection of si-FTO in injured cells could promote its effects. N-O Flow cytometry assays were performed to determine the apoptotic rate in different groups. The results showed that cells’ apoptotic rate was increased in the cisplatin-induced injured cell, and co-transfection of si-FTO could promote it. One representative experiment of three independent experiments is shown. The results represent the mean ± SEM from three independent experiments. Student’s t-test and one-way ANOVA were applied to compare the two experimental and multiple groups, respectively. (*P < 0.05, **P < 0.01, ***P < 0.001)

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