Fig. 3

MenSCs restored the expression of FTO in injured granulosa cells and POF mice. A-C qRT-PCR and western blotting results showed that FTO expression was decreased in injured granulosa cells, and MenSCs co-culture could restore it. The results represent the mean ± SEM from three independent experiments. D-G POF mice models and MenSCs transplantation were established, and changes in the body weight, serum levels of E2, FSH, and AMH in different groups were shown. The graphs show the mean ± SEM (n = 15 mice per group). H H & E staining showed the morphological, follicle numbers, and histopathological change of the ovary after different treatments. I IHC results showed the FTO expression was decreased in the POF group compared to the controls, while MenSCs transplantation could restore it. (n = 15 mice per group). Scale bars in the HE staining represents 8 × 200 μm, while in the IHC staining represents 15 × 200 μm. Student’s t test. (*P < 0.05, **P < 0.01, ***P < 0.001)