Fig. 2
From: FTO protects human granulosa cells from chemotherapy-induced cytotoxicity
Effects of MenSCs and conditional medium (CM) on injured granulosa cells apoptosis and proliferation in vitro. A-B qRT-PCR and C-E western blotting indicated that MenSCs coculture with the injured granulosa cells could improve the injured granulosa cell anti-apoptosis ability by increasing the expression of Bcl-2 and decreasing the expression of BAX at the transcriptional and protein levels. One representative experiment of three independent experiments is shown. F A CCK-8 assay was used to measure the effect of condition medium (CM) obtained from the MenSCs on the in injured granulosa cells’ proliferation. The results showed that the CM could promote the injured granulosa cell viability significantly. β-actin served as the loading control. The results represent the mean ± SEM from three independent experiments. Student’s t test. (*P < 0.05, **P < 0.01, ***P < 0.001)