Fig. 1

The effect of cisplatin on granulosa cells proliferation and apoptosis. A a CCK-8 assay was used to measure the effect of cisplatin on granulosa cell viability. Cisplatin inhibits granulosa cell proliferation rate in a dose-dependent and time-dependent manner. B-F qRT-PCR and western blotting showed that when the concentration of cisplatin was lower than 10 µM, as the concentration of cisplatin increased, the apoptotic ability of cells gradually increased, while the anti-apoptotic ability gradually weakened. When the cisplatin concentration was higher than 10 µM, the opposite result was obtained. One representative experiment of three independent experiments is shown. Data are shown as the mean ± SD from three independent experiments. β-actin served as the loading control. (*p < 0.05; **p < 0.01; ***p < 0.001 by Student’s t test. CCK-8: cell counting kit‐8)