Fig. 6
From: Down-regulation of PBK inhibits proliferation of human endometrial stromal cells in thin endometrium
The contribution of oxidative stress, hypoxia and inflammatory factors to PBK downregulation. A The mRNA level of PBK in HESCs treated with different concentrations of hydrogen peroxide for 48 h (n = 3). B The protein levels of PBK, CDK1 and CCNB1 were detected by western blot after 48 h. C The mRNA level of PBK in HESCs under hypoxia condition with 1% O2 at different time points (n = 4). D The protein levels of PBK, CDK1 and CCNB1 were detected by qPCR after 24 h or 48 h. E RNA-seq results showed that IL-1β (10 ng/mL) in endometrium samples from TE patients was upregulated compared with that in controls. Fold change = 2.45, P-value = 0.0398. F The mRNA level of PBK in HESCs stimulated with 10 ng/ml IL-1β at different time point (n = 4). G The protein levels of PBK, CDK1 and CCNB1 were detected by western blot after incubation with 10 ng/ml IL-1β for 24 h or 48 h. The results were representative of three independent experiments. All quantified data are presented as mean ± SEM; **P < 0.01, ***P < 0.001. TE: thin endometrium