Fig. 5

Kisspeptin mediates TGF-β1-suppressed human EVT cell invasion. A and B HTR-8/SVneo cells were treated with 5 ng/mL TGF-β1 (A) or 1 μM kisspeptin (B) and then seeded onto Matrigel-coated transwell inserts. The levels of cell invasiveness were examined by transwell invasion assay. C and E HTR-8/SVneo (C) and primary human EVT (E) cells were transfected with 50 nM control siRNA (si-Ctrl) or kisspeptin siRNA (si-KISS1) for 48 h, and then treated with 5 ng/mL TGF-β1 for 24 h. The kisspeptin protein levels were examined by western blot. D and F HTR-8/SVneo (D) cells and primary human EVT (F) cells were transfected with 50 nM control siRNA (si-Ctrl) or kisspeptin siRNA (si-KISS1) for 48 h and then treated with 5 ng/mL TGF-β1. The levels of cell invasiveness were examined by transwell invasion assay. Original magnification: 100x. The scale bar represents 50 μm. The results are expressed as the mean ± SEM of at least three independent experiments. Values that are statistically different from one another (p < 0.05) are indicated by different letters