Fig. 6

Knockdown of Zip12 decreased zinc content and the antioxidant capacity of C18–4 cells. A Western blot analysis of ZIP12 protein expression levels in the control and the ZIP12 shRNA transfected group. The relative levels of ZIP12 protein were normalized to β-actin. Data are shown presented as means ± SEM from three independent experiments. B-C Flow cytometric analysis of the fluorescence intensity of zinc on the control (n = 5) and the ZIP12 shRNA transfected group (n = 5). B One representative sample showed P2 region, which represented cells transfected with control shRNA or ZIP12-specific shRNA that were positive for GFP signal. C Mean fluorescence intensity of zinc of the two groups indicated a decrease in the zinc level in ZIP12-knock down C18–4 cells. Data were presented as means ± SEM from four independent experiments. D CCK-8 proliferation assay in control shRNA (n = 4) or ZIP12-specific shRNA (n = 4) transfected C18–4 cells with the treatment of 100 μM H₂O₂. Data are shown as means ± SEM from four independent experiments. E Relative Caspase-3 activity in C18–4 cells transfected with control shRNA or ZIP12-specific shRNA and treated with 100 μM H₂O₂. Data are shown as means ± SEM from three independent experiments. *P < 0.05, ****P < 0.0001