Fig. 5

ZIP12 expression in response to oxidative stress in the C18–4 spermatogonia cell line. A Immunofluorescent staining of ZIP12 (red) in C18–4 cells. Cell nuclei were stained with DAPI (blue). Scale bar = 50 μm. B Western blot detection of ZIP12 protein expression levels in C18–4 cells after 100 μM H₂O₂ treatment for 24 h, 48 h, 72 h. The relative levels of ZIP12 protein were normalized to β-actin. Data are shown presented as means ± SEM from three independent experiments. C Western blot detection of ZIP12 protein expression levels in C18–4 cells after 50 μM PA treatment for 24 h and 48 h. The relative levels of ZIP12 protein were normalized to β-actin. Data are presented as means ± SEM from six independent experiments. *P < 0.05, **P < 0.01, ****P < 0.0001