Fig. 4

Comparison of sperm parameters, ROS level, and ZIP12 expression in obese mice. A Comparison of time-dependent increases in body weight between CD (n = 10) and HFD groups (n = 10). B Sperm motility in mice fed either CD or HFD analyzed by CASA (n = 10). C Sperm morphology analysis of CD-fed mice and HFD-fed mice using Diff-Quick method (n = 10). D Comparison of sperm ROS level in CD and HFD groups (n = 7). E Fluorescent intensity of Zinc in mouse sperm stained by ZnAF-1 and analyzed by flow cytometry (n = 5). F Western blot detection of ZIP12 protein in CD (n = 3) and HFD (n = 8) mice. The relative levels of ZIP12 protein were normalized to β-actin. Data are shown as means ± SEM. *P < 0.05, **P < 0.01, ****P < 0.0001