Fig. 6

MiR-423-5p regulated TTR-induced KGN cell disorders through MTM1. A and B The effects between miR-423-5p and MTM1 on MTM1 expression were determined by qRT-PCR and Western blot analysis. C-H KGN cells were treated with TTR, TTR + miR-NC, TTR + miR-423-5p, TTR + miR-423-5p + pcDNA or TTR + miR-423-5p + MTM1 with mock KGN cells as a control, and cell proliferation was analyzed by EdU, MTT and flow cytometry analysis for cell cycle assays (C-E), the protein expressions of PCNA, Bcl-2, Bax and Cleaved PARP by Western blot (F and H), and cell apoptosis by flow cytometry analysis for cell apoptosis (G). Statistical significances were compared by one-way analysis of variance with Tukey’s test. *P < 0.05