Fig. 5

Circ_FURIN regulated MTM1 expression by associating with miR-423-5p. A The complementary sites of miR-423-5p with MTM1. B and C Dual-luciferase reporter and RNA pull-down assays were performed to identify the binding relationship of miR-423-5p and MTM1. D The efficiency of miR-423-5p overexpression and knockdown was analyzed by qRT-PCR. E and F The impacts of miR-423-5p overexpression and depletion on MTM1 expression were investigated by qRT-PCR and Western blot. G and H The mRNA and protein expressions of MTM1 were checked by qRT-PCR and Western blot, respectively, in the lesion ovarian cortex tissues of PCOS patients and healthy ovarian cortex tissues. I and J The linear correlation between MTM1 and miR-423-5p or circ_FURIN was analyzed by Spearman correlation analysis. K and L MTM1 expression was detected by qRT-PCR and Western blot in TTR-treated KGN cells and mock KGN cells. M and N The impacts between circ_FURIN knockdown and miR-423-5p depletion on MTM1 expression were investigated by qRT-PCR and Western blot. NS: no significant difference. Statistical significances were compared by Student’s t-tests in (B, C, K and L), one-way analysis of variance with Tukey’s test (D, E, F, M and N), Wilcoxon rank-sum test in (G and H), and Spearman’s correlation test in (I and J). *P < 0.05