Fig. 3

Circ_FURIN was associated with miR-423-5p. A The schematic illustration showing the complementary sites of circ_FURIN with miR-423-5p. B and C Dual-luciferase reporter and RNA pull-down assays were performed to analyze the interplay between circ_FURIN and miR-423-5p. D The effect of circ_FURIN depletion on miR-423-5p expression was determined by qRT-PCR. E Spearman correlation analysis was conducted to clarify the correlation of circ_FURIN and miR-423-5p in the ovarian cortex tissues of PCOS patients. F and G MiR-423-5p expression was detected by qRT-PCR in mock KGN cells, the KGN cells treated with TTR (10 μM), lesion ovarian cortex tissues of PCOS patients (N = 21) and healthy ovarian cortex tissues (N = 13). NS: no significant difference. Statistical significances were compared by Student’s t-tests in (B, C, D and F), Spearman’s correlation test in (E), and Wilcoxon rank-sum test in (G). *P < 0.05