Skip to main content
Fig. 1 | Reproductive Biology and Endocrinology

Fig. 1

From: GnRH antagonist weakens endometrial stromal cells growth ability by decreasing c-kit receptor expression

Fig. 1

Comparison of the growth ability of human isolated ESCs after treating with different concentrations of GnRH-ant and GnRH-a (10− 8, 10− 5 and 2 × 10− 5 mol/L). A The proliferation of ESCs was investigated by using CCK8 assay. No difference was found in the proliferation of ESCs at concentration of 10− 8 and 10− 5 mol/L between GnRH-ant and GnRH-a group. However, when the concentration was added up to 2 × 10− 5 mol/L, the proliferation capacity of ESCs in GnRH-ant group was significantly restrained compared to those in GnRH-a group. B Flow cytometry was used to analyze the apoptosis of ESCs. Similarly, the apoptotic level of ESCs was remarkably increased when the concentration at 2 × 10− 5 mol/L. Each group of experiment was repeated for three times. (Control group: ESCs was treated only with culture medium. GnRH-ant-control group: ESCs was treated with GnRH-ant solvent. GnRH-a-control group: ESCs was treated with GnRH-a solvent.) (*** p < 0.001)

Back to article page

Reproductive Biology and Endocrinology

ISSN: 1477-7827

Contact us