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Fig. 4 | Reproductive Biology and Endocrinology

Fig. 4

From: Homozygous mutation in SLO3 leads to severe asthenoteratozoospermia due to acrosome hypoplasia and mitochondrial sheath malformations

Fig. 4

The acrosome reaction and expression patterns of acrosome are changed in the spermatozoa from SLO3-mutant man. A Influence of the SLO3 mutation on spontaneous acrosome reaction. Staining with PSA-FITC: acrosome reacted spermatozoa (red asterisk); non-acrosome reacted spermatozoa (red arrowhead). B Percentage of spontaneous acrosome reaction. Data are presented as the mean ± SEM. Bars represent standard error. Significant differences, ***P < 0.001; Student’s t-test. C Peanut agglutinin (PNA) staining conjugated to rhodamine for the localization patterns of spermatozoa membrane galactose. D Localization patterns of PLCζ1 in normal and mutant sperm. Representative images of spermatozoa from controls and the SLO3-mutated individual stained with anti-PLCζ1 antibody, anti-Ac-tubulin antibody, and Hoechst. PLCζ1 staining is concentrated at the acrosomal region in control sperm, but absent in most mutant sperm, Scale bars: 20 μm

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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