Fig. 2

Size distribution, concentration, and morphology of EVs secreted by Ishikawa cells isolated by different methodologies. A Representative set of NTA plots of EVs isolated by the Norgen kit, the ExoQuick-TC reagent, or ultracentrifugation, with a dilution factor of 1:100 for Norgen-isolated samples and 1:1,000 for samples isolated by ExoQuick-TC and ultracentrifugation. B Summary table of the size distribution and concentration data of isolated nanoparticles. C Representative set of TEM images showing the morphology of EVs isolated by the three methodologies with a 200-nm and D 500-nm magnification, with total counts of artifacts present in the samples, arrowheads show artifacts; n = 3 replicates/method; ***P < 0.001; **P < 0.01; *P < 0.05; ns, not significant. Data are presented as Mean ± SD