Fig. 6

Involvement of p65 in SAA1-induced insulin resistance in cultured granulosa cells. A, B Effect of SAA1 (1 μg/ml for 15, 30, 60 and 180 min) on the phosphorylation of p65 (n = 4). ** P < 0.01 compared with ctr (0), # P < 0.05 compared with 60 min. C, D Effect of OxpAPC (30 μg/ml) on SAA1 (1 μg/ml for 60 min) induced p65 phosphorylation (n = 4). * P < 0.05 compared with control, # P < 0.05 compared with SAA1. E, F Effect of JSH-23 (10 μM), a p65 inhibitor, on SAA1 (1 μg/ml for 24 h) induced PTEN expression (n = 3). * P < 0.05 compared with control, # P < 0.05 compared with SAA1. G, H Effect of JSH-23 (10 μM) on SAA1 (1 μg/ml for 24 h) -attenuated Akt phosphorylation induced by insulin (100 nM for 15 min) (n = 4). * P < 0.05 compared with control, # P < 0.05 compared with SAA1. I, J Effect of JSH-23 (10 μM) on SAA1 (1 μg /ml for 24 h) -attenuated GLUT4 translocation induced by insulin (100 nM for 30 min) (n = 3). ** P < 0.05 compared with control, # P < 0.05 compared with SAA1. K Effect of JSH-23 (10 μM) on SAA1 (1 μg /ml for 24 h) -attenuated glucose uptake induced by insulin (100 nM for 30 min) (n = 4). * P < 0.05 compared with control, # P < 0.05 compared with SAA1. All values are the mean ± SEM