Fig. 5

Role of TLR2/4 in SAA1-induced insulin resistance in cultured granulosa cells. A, B Effect of OxpAPC (30 μg/ml), a TLR2/4 inhibitor, on SAA1 (1 μg/ml for 24 h)-induced PTEN protein expression (n = 3). C, D Effect of OxpAPC on SAA1(1 μg/ml for 24 h)-attenuated Akt phosphorylation induced by insulin (100 nM for 15 min)(n = 4). E, F Effect of OxpAPC (30 μg/ml) on SAA1 (1 μg /ml for 24 h) -attenuated GLUT4 translocation induced by insulin (100 nM for 30 min) (n = 4). G Effect of OxpAPC (30 μg/ml) on SAA1 (1 μg /ml for 24 h) -attenuated glucose uptake induced by insulin (100 nM for 30 min) (n = 3). All values are the mean ± SEM. * P < 0.05 compared with control, # P < 0.05 compared with SAA1