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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Increased METTL3-mediated m6A methylation inhibits embryo implantation by repressing HOXA10 expression in recurrent implantation failure

Fig. 3

METTL3 epigenetically decreases HOXA10 expression. a The mRNA levels of HOXA10 in the endometrial tissues from RIF patients (n=13) and normal control women (n=13) were evaluated by qRT-PCR. b The methylation of HOXA10 mRNA in the wild-type/mutant (D395A and W398A) METTL3-overexpressing Ishikawa cells were analyzed by Me-RIP-qRT-PCR. c-d The mRNA and protein levels of HOXA10 in the wild-type/mutant (D395A and W398A) METTL3-overexpressing Ishikawa cells were analyzed by qRT-PCR (c) and Western blotting (d), respectively. e The curve and statistical analysis of HOXA10 mRNA decay slope in the wild-type/mutant (D395A and W398A) METTL3-overexpressing Ishikawa cells after transcriptional inhibition. *P<0.05, ***P<0.001 versus the indicated group. N.S. means P >0.05

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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