Fig. 5

The phosphorylation of p4E-BP1, p70S6kinase and S6 ribosomal protein in ESCs treated with AICAR (3 mM) is shown (n = 4). The phosphorylation of p4E-BP1, p70S6K and S6 ribosomal protein was quantified against GAPDH. A: Representative blots illustrating the effect of treatment on p4EBP-1 and GAPDH by AICAR (3 mM) for 0–240 min (n = 4). The blot layout is in the same order as the graph in panels B (4EBP-1), C (p70S6K), D (S6 ribosomal protein), respectively. *P < 0.001 vs. controls