Fig. 4

The phosphorylation of IκB in ESCs treated with IL-1β with or without AICAR (n = 4). GAPDH is shown as the internal control. A: Representative blots illustrating the effect of IL-1β (1 nM) treatment on IκB and GAPDH for 0–240 min. The blot layout is in the same order as the graph in panels B. C: Representative blots illustrating the effect of IL-1β (1 nM) treatment and/or pretreatment with AICAR (0.3-3 mM) for 15 min on IκB and GAPDH. The blot layout is in the same order as the graph in panel D. **P < 0.01 vs. IL-1β only. ***P < 0.001 vs. IL-1β only