Fig. 4

Identification of relevant factor by cytokine array. (A) Venn diagram of cytokines which were up-regulated in more than two out of three patients in the GnRHa group and down-regulated in more than two out of three patients in the DNG group in comparison with the time point of entry and the time point of 4 months of treatment. The roster of cytokines that are common in both groups is shown on the right side. (B) The ratio of serum IL-6 levels is defined as [4 M post-treatment IL-6 level / pre-treatment IL-6 level]. In the box plot, center lines show medians; box limits indicate the 25th and 75th percentiles; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles. n.s. stands for not significant. Data were analyzed by two-tailed Student’s t-test. (C) The number of patients whose ratio of serum IL-6 levels was under 0.5 or over 0.5 in each group. The P-value was calculated using the Chi-square test. (D) Scatter plot showing the correlation between AMH and IL-6 rate in each group. (GnRHa; r=-0.05, P<0.01, DNG; r=-0.77, P=0.23) The r is the Pearson correlation coefficient