Fig. 1

Analysis of MAIT cells and MDSCs in peripheral blood from patients by flow cytometry. Representative pseudocolor with the gating strategy identifying CD8⁺, CD4⁺, DN MAIT (A). CD3⁺CD161⁺ Vα7.2⁺ cells were gated at first. Basing on CD4 and CD8, and three subsets were identified: CD8⁺, CD4⁺ and DN MAIT cells. At last, cells which had expression of CD38 (CD38⁺) were selected. The gating strategy identifying MDSCs (B). Firstly, CD33⁺ cells were gated and then HLA-DR^-/low and CD11b⁺ cells were isolated. Next, based on CD14 and CD15, the cells were divided into Mo-MDSC (CD14⁺CD15^-) and PMN-MDSC (CD14^-CD15⁺). MAIT cells were positively correlated with MDSCs (C). MAIT cell, mucosal-associated invariant T cell; MDSC, myeloid-derived suppressor cell; DN, double negative; Mo-MDSC, monocytic myeloid-derived suppressor cell; PMN-MDSC, polymorphonuclear myeloid-derived suppressor cell